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(AT16495)His-Tag

产品编号: AT16495M/AT16495S

产品规格: 50μL 20μL


  

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  • (AT16495)His-Tag 说明书

    Description

    Product Name:              His-Tag antibody

    Host Species:                Mouse

    Clonality:                      Monoclonal antibody (mAb)

    Purification:                 Monoclonal antibody is purifed by affnity isolation.

    Applications:               Western bloting; Immunofluorescence (IF); Immunocytochemistry (ICC)

    Species Reactivity:       N/A

    Specificity:                  His-Tag mouse mab detects endogenous levels of internal,

                                        C-terminal, or N-terminal 6His-tagged proteins.

    Immunogen Type:      Peptide-KLH

    Immunogen Description:

                                        A synthetic peptide HHHHH coupled to KLH.

    Target Name:             His-Tag

    concentration:           1.0mg/ml

    Storage:                    Store at -20℃ for long term preservation (recommeded).

                                       Store at 4℃  for short term use.

    Note:                        In order to get the best results using various techniques and preparations,

                                       we recommend detecting optimal working dilutions by titration.

  • Application Details

    SDS-PAGE molecular Wt: N/A

    Western blotting: 1:1000~1:3000 (recommended 1:2000)

    Immunofluorescence/ Immunocytochemistry: 1:50~1:200

  • Images

    IP antibody use: 5 ug His mouse IgG1 per ml lysate.

    Western blot analysis using His-tag mouse mAb antibody #AT16495.1 Untransfected 293 cell lysate

    2. Transfected 293 cell lysate with His-tag fusion protein

    3. IP (untransfected 293 + anti-His mAb + protein G agarose)

    4. IP (transfected 293 + normal mouse lgG + protein G agarose)

    5. IP (transfected 293 + anti-His mAb+ protein G agarose)

    6. Recombinant protein (E coli)